In this study, an attempt was made to measure the chemotatic activity of PMNs in Salmonellosis. Each 15 ml of heparinized blood was obtained from 6 patients of typhoid fever in the Northwick Park Hospital and Clinical Research Centre from June in 1978 to Novemer.
Each 1 ml of 3% dextran was added and the treated blood was incubated at 37°C for 4C minutes. The supernatant was harvested, washed two times with TC-199 medium, and the concentration of cells was adjusted at I X 10´ ml. The casein (0.5 %) was used as a chemotactic factor with Boyden chamber modified by Larson (1977) used. The pores of the membrane millipore filters were 0.8pm and 0, 45pm in diameter (filter type SC made in France). The chamber inserted filters was filled with 0.4 ml of PMNs and kept at 37°C for 2. +hrs.
Then, picked out the filter, we stained it with Harris haematoxicillin and counted only five fields through the microscope.
The results were as follows:
1. The chemotactic activity of PMNs in control had no significant differences.
2. The longer the measuring time was, the more migrated the cells were. But, in this study a chamber was activated for 2. + hrs.
3. In typhoid fever, the chemotactic activity of PMNs appeared with a remarkable difference between one early infected stage and the other convalescent. Namely, the reactivity of the early infected stage was lower than that of the convalescent one.
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